Open AccessCu/Zn superoxide dismutase in yeast mitochondria – a general phenomenon
Author(s) -
Nedeva Trayana S,
Petrova Ventzislava Y,
Zamfirova Daniela R,
Stephanova Elena V,
Kujumdzieva Anna V
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00855-3
Subject(s) - yeast , superoxide dismutase , mitochondrion , kluyveromyces , cytosol , dismutase , biochemistry , saccharomyces cerevisiae , chemistry , biology , enzyme
Fermentative and respiratory yeast strains of genera Saccharomyces , Kluyveromyces , Pichia , Candida and Hansenula have been investigated for mitochondrial localization of Cu/Zn superoxide dismutase (SOD). Pure mitochondrial fractions were obtained and the specific activities of Cu/Zn and Mn SODs were measured in comparison with those in the corresponding cell‐free extracts. The Cu/Zn SOD: Mn SOD ratio in mitochondria and crude extracts was calculated and was considered a specific characteristic of all tested strains. Electrophoretical visualization of SOD patterns provided evidence for possible migration of cytosolic Cu/Zn SOD to mitochondria. The characteristic Cu/Zn SOD profile in mitochondria of all tested strains suggested its ubiquity within the fermentative and respiratory yeasts.