Open AccessStreptococcus alactolyticus is the dominating culturable lactic acid bacterium species in canine jejunum and feces of four fistulated dogs
Author(s) -
Rinkinen Minna L.,
Koort Joanna M.K.,
Ouwehand Arthur C.,
Westermarck Elias,
Björkroth K.Johanna
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00851-6
Subject(s) - biology , lactobacillus reuteri , feces , serial dilution , microbiology and biotechnology , 16s ribosomal rna , bacteria , lactobacillus , agar , lactobacillus gasseri , ribotyping , population , polymerase chain reaction , pathology , gene , demography , sociology , medicine , biochemistry , genetics , alternative medicine
Canine intestinal lactic acid bacterium (LAB) population in four fistulated dogs was cultured and enumerated using MRS agar. LAB levels ranging from 1.4×10 6 to 1.5×10 7 CFU ml −1 were obtained in jejunal chyme. In the fecal samples 7.0×10 7 and 2.0×10 8 CFU g −1 were detected. Thirty randomly selected isolates growing in the highest sample dilutions were identified to species level using numerical analysis of 16S and 23S rDNA restriction fragment length polymorphism patterns (ribotyping) and 16S rDNA sequence analysis. According to these results, Streptococcus alactolyticus was the dominant culturable LAB species in both feces and jejunal chyme. In addition, Lactobacillus murinus and Lactobacillus reuteri were detected.