Open AccessThe use of amplified fragment length polymorphism for molecular analysis of Beauveria bassiana isolates from Kenya and other countries, and their correlation with host and geographical origin
Author(s) -
Aquino de Muro Marilena,
Mehta Samina,
Moore David
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00841-3
Subject(s) - beauveria bassiana , amplified fragment length polymorphism , biology , restriction fragment length polymorphism , beauveria , internal transcribed spacer , genetics , genotype , bassiana , ribosomal dna , terminal restriction fragment length polymorphism , polymorphism (computer science) , spacer dna , ribosomal rna , botany , genetic diversity , phylogenetics , gene , biological pest control , population , demography , sociology
Beauveria spp. (one Beauveria sp., two B. brongniartii , and 47 B. bassiana ) isolated from insects and soil from Kenya and other 16 different countries, were obtained from the CABI Bioscience Genetic Resource Collection. DNA was extracted from the 50 isolates and their genetic variability was investigated using restriction analysis of the internal‐transcribed‐spacer ribosomal region restriction fragment length polymorphism (ITS‐RFLP), ITS sequencing, and amplified fragment length polymorphism (AFLP). The B. bassiana isolates could not be distinguished by ITS‐RFLP as all of them showed the same banding pattern. However, the AFLP technique provided more information on polymorphism between the isolates, allowing them to be clustered by relative similarity using band matching and unweighted pair group method with arithmetic mean analysis. Although no significant correlation between the isolates and host and geographical origins were observed, the technique revealed clonal populations of B. bassiana within Kenya.