Open AccessConstruction of vectors for inducible gene expression in Lactobacillus sakei and L. plantarum
Author(s) -
S?rvig Elisabeth,
Grönqvist Sonja,
Naterstad Kristine,
Mathiesen Geir,
Eijsink Vincent G.H.,
Axelsson Lars
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00798-5
Subject(s) - lactobacillus sakei , lactococcus lactis , biology , lactobacillus plantarum , bacteriocin , gene , expression vector , restriction enzyme , reporter gene , operon , nisin , microbiology and biotechnology , gene expression , lactobacillus , genetics , bacteria , escherichia coli , recombinant dna , lactic acid
We have constructed vectors for inducible expression of genes in Lactobacillus sakei and Lactobacillus plantarum . The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and sakacin P and the genes encoding the cognate histidine protein kinase and response regulator that are necessary to activate this promoter upon induction by a peptide pheromone. The vectors are built up of cassettes that permit easy exchange of all parts through restriction enzyme digestion and ligation. Using β‐glucuronidase as a reporter enzyme, variants of these vectors were compared with each other, and with a corresponding system based on genes involved in the production of nisin. Several of the new vectors permitted tightly controlled and efficient expression of β‐glucuronidase in both L. sakei and L. plantarum .