Open AccessMice immunization with live lactococci displaying a surface anchored HPV‐16 E7 oncoprotein
Author(s) -
CortesPerez Naima G,
BermúdezHumarán Luis G,
Loir Yves,
RodriguezPadilla Cristina,
Gruss Alexandra,
SaucedoCárdenas Odila,
Langella Philippe,
MontesdeOcaLuna Roberto
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00778-x
Subject(s) - lactococcus lactis , nisin , recombinant dna , microbiology and biotechnology , bacteria , immune system , biology , immunization , virology , immunology , antimicrobial , gene , lactic acid , biochemistry , genetics
E7 oncoprotein of human papillomavirus‐16 (HPV‐16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin‐controlled expression system was used to display the E7 protein at the cell surface of the food‐grade Gram‐positive bacterium Lactococcus lactis . An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV‐16 E7‐specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food‐grade bacteria to fight against CxCa.