Open AccessImproved flagellin genotyping in the Burkholderia cepacia complex
Author(s) -
Winstanley Craig
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00721-3
Subject(s) - biology , burkholderia cepacia complex , burkholderia cenocepacia , flagellin , polymerase chain reaction , burkholderia , genotyping , amplicon , microbiology and biotechnology , gene , genetics , typing , genotype , bacteria
Oligonucleotide primers designed to N‐ and C‐terminal sequences of Burkholderia cepacia complex fliC genes were used to amplify and sequence fliC genes from a strain of Burkholderia vietnamiensis and three isolates of Burkholderia multivorans with large fliC genes. Alignments incorporating the new sequences enabled the design of polymerase chain reaction (PCR) primers for extension of a published PCR/restriction fragment length polymorphism typing method, to include isolates that previously failed to yield fliC amplicons. Most B. vietnamiensis isolates and hitherto non‐typable Burkholderia cenocepacia isolates contained much smaller fliC genes than previously reported. Although B. multivorans strains with larger fliC genes clustered together, relationships between strains based on fliC sequences did not generally correlate with genomovar status.