Open AccessCbiX‐homologous protein (CbiXhp), a metal‐binding protein, from Streptomyces seoulensis is involved in expression of nickel‐containing superoxide dismutase
Author(s) -
Kim InKwon,
Yim YangIn,
Kim YoungMin,
Lee JinWon,
Yim HyungSoon,
Kang SaOuk
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00719-5
Subject(s) - streptomyces coelicolor , bacillus megaterium , biochemistry , superoxide dismutase , chemistry , streptomyces , binding protein , nickel , microbiology and biotechnology , biology , bacteria , gene , enzyme , genetics , mutant , organic chemistry
To find the accessory proteins participating in expression and maturation of nickel‐containing superoxide dismutase (NiSOD), a metal‐binding protein (CbiXhp) homologous to cobaltochelatase (CbiX) of Bacillus megaterium was isolated by nickel‐nitrilotriacetic acid resin from Streptomyces seoulensis . The deduced amino acid sequence of cbiXhp showed 87% and 39% identity to CbiX of Streptomyces coelicolor and that of B. megaterium , respectively. Overexpression of CbiXhp increased the activity and the expression of NiSOD in the presence and absence of nickel, but to a lesser extent in its absence. This result indicates that CbiXhp is involved in the expression of NiSOD.