Open AccessEffects of metronidazole and tinidazole on NTPDase1 and ecto‐5′‐nucleotidase from intact cells of Trichomonas vaginalis
Author(s) -
Tasca Tiana,
Borges Fernanda Pires,
Bonan Carla Denise,
Carli Geraldo Attilio,
Battastini Ana Maria Oliveira,
Sarkis João José Freitas
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00637-2
Subject(s) - tinidazole , metronidazole , trichomonas vaginalis , atp hydrolysis , microbiology and biotechnology , chemistry , adenosine , extracellular , hydrolysis , adenosine triphosphate , biochemistry , enzyme , biology , antibiotics , atpase
Here we report the effects of metronidazole and tinidazole on NTPDase1 and ecto‐5′‐nucleotidase from intact cells of Trichomonas vaginalis . Adenosine triphosphate (ATP) and adenosine diphosphate (ADP) hydrolysis was 5‐ to 7‐fold higher for the fresh clinical strain, when compared with the ATCC (American Type Culture Collection) strain. ATP hydrolysis was activated in the presence of metronidazole in the ATCC strain, whilst it was inhibited 33% by 50 μM tinidazole in a fresh clinical isolate. The treatment of cells in the presence of metronidazole for 2 h inhibited ATP and ADP hydrolysis, whilst treatment with tinidazole inhibited ATP and ADP hydrolysis only in the fresh clinical isolate. The drugs did not change the ecto‐5′‐nucleotidase activity for both strains. Our results suggest that the modulation of extracellular ATP and ADP levels during treatment with these drugs could be a parasitic defence strategy as a survival mechanism in an adverse environment.