Open AccessIdentification of rpoE and nadB as host responsive elements of Yersinia enterocolitica
Author(s) -
Heusipp Gerhard,
Schmidt M.Alexander,
Miller Virginia L.
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00613-x
Subject(s) - yersinia enterocolitica , microbiology and biotechnology , identification (biology) , host (biology) , yersinia , biology , enterobacteriaceae , bacteria , genetics , escherichia coli , gene , ecology
We describe the identification of the Yersinia enterocolitica rpoE gene, which encodes the alternative sigma factor σ E , and the divergently transcribed nadB gene, as genes that are expressed during infection of mice. As in Escherichia coli , rpoE of Y. enterocolitica is essential for growth and its expression is autoregulated. Despite the similarities of the rpoE operons of Y. enterocolitica and E. coli , there are considerable differences in the response to extracytoplasmic stress. Unlike in E. coli , σ E of Y. enterocolitica is not induced by heat shock or ethanol. Overproduction of the outer membrane protein Ail does not lead to the induction of rpoE expression. However, rpoE expression is induced by osmotic stress.