Open AccessTwo phenotypically compensating isocitrate dehydrogenases in Ralstonia eutropha
Author(s) -
Wang ZhengXiang,
Brämer Christian,
Steinbüchel Alexander
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00612-8
Subject(s) - glyoxylate cycle , isocitrate dehydrogenase , idh1 , isocitrate lyase , citric acid cycle , idh2 , biochemistry , biology , enzyme , mutant , chemistry , gene
The tricarboxylic acid (TCA) cycle enzyme isocitrate dehydrogenase (IDH) and the glyoxylate bypass enzyme isocitrate lyase are involved in catabolism of isocitrate and play a key role in controlling the metabolic flux between the TCA cycle and the glyoxylate shunt. Two IDH genes icd1 and icd2 of Ralstonia eutropha HF39, encoding isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2), were identified and characterized. Icd1 was functionally expressed in Escherichia coli , whereas icd2 was expressed in E. coli but no activity was obtained. Interposon‐mutants of icd1 (HF39Δ icd1 ) and icd2 (HF39Δ icd2 ) of R. eutropha HF39 were constructed and their phenotypes were investigated. HF39Δ icd1 retained 43% of IDH activity, which was not induced by acetate, and HF39Δ icd2 expressed 74% of acetate‐induced IDH activity. Both HF39Δ icd1 and HF39Δ icd2 kept the same growth rate on acetate as the wild‐type. These data suggested that IDH1 is induced by acetate. The interposon‐mutants HF39Δ icd1 and HF39Δ icd2 accumulated the same amount of poly(3‐hydroxybutyric acid) as the wild‐type.