Open AccessIsolation, characterization and expression of a cyclin from Leishmania donovani
Author(s) -
Banerjee Sampali,
Banerjee Rahul,
Das Ruma,
Duttagupta Swadesh,
Saha Partha
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00606-2
Subject(s) - biology , leishmania donovani , open reading frame , microbiology and biotechnology , complementary dna , cyclin , northern blot , dna , cdna library , cyclin b1 , leishmania , cell cycle , peptide sequence , genetics , gene , cyclin dependent kinase 1 , parasite hosting , visceral leishmaniasis , leishmaniasis , world wide web , computer science
We have cloned and sequenced a DNA fragment (∼1 kb) containing a complete open reading frame from a cDNA library of Leishmania donovani promastigotes. The alignment of the derived polypeptide sequence and the modeling studies revealed that the protein is highly homologous to the mammalian cyclins having conserved cyclin box and substrate‐docking motif. Northern blot analysis of the RNA isolated from synchronized L. donovani promastigotes showed periodic expression of the message with maximum abundance at S‐phase suggesting its involvement in the events related to the regulation of DNA replication. The results confirm that we have isolated a cyclin molecule from L. donovani (LdCyc1) which may play an important role in the regulation of the parasite cell cycle.