Open AccessTranscriptional control of a rRNA promoter of the nodulating symbiont Sinorhizobium meliloti
Author(s) -
Rosado Michelle,
Gage Daniel J.
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00603-7
Subject(s) - sinorhizobium meliloti , biology , plasmid , transcription (linguistics) , exponential growth , green fluorescent protein , sinorhizobium , strain (injury) , microbiology and biotechnology , bacteria , gene , rhizobiaceae , genetics , symbiosis , physics , linguistics , philosophy , anatomy , quantum mechanics
We constructed a stable, low‐copy‐number plasmid containing a fusion between a Sinorhizobium meliloti rRNA promoter and gfp(mut3) . When transformed into S. meliloti the resulting strain, Rm1021/pKW1, fluoresced in proportion to its growth rate during balanced growth. This strain also showed an unexpected behavior when grown to stationary phase in TY medium: the average cellular fluorescence increased through mid‐exponential phase then decreased dramatically. The explanation for this appears to be that transcription from the rRNA promoter was shut off in mid‐exponential phase and intracellular Gfp was diluted by continued cell growth.