Open AccessLocalizing cell division in spherical Escherichia coli by nucleoid occlusion
Author(s) -
Zaritsky Arieh,
Woldringh Conrad L
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00580-9
Subject(s) - nucleoid , ftsz , escherichia coli , cell division , dapi , biology , fluorescence microscope , thymine , chromosome segregation , microbiology and biotechnology , dna replication , dna , biophysics , cell , chemistry , genetics , fluorescence , chromosome , physics , gene , optics , staining
The spatial relationship between FtsZ localization and nucleoid segregation was followed in Escherichia coli thyA cells, made spheroidal by brief exposure to mecillinam and after manipulating chromosome replication time using changes (‘steps’) in thymine concentration [Zaritsky et al., Microbiology 145 (1999) 1015–1022]. In such cells, fluorescent FtsZ‐GFP arcs did not overlap the DAPI‐stained nucleoids. It is concluded that FtsZ rings are deposited between segregating nucleoids, consistent with the nucleoid occlusion model [Woldringh et al., J. Bacteriol. 176 (1994) 6030–6038].