Open AccessMicromanipulation of the Chlamydia pneumoniae inclusion: implications for cloning and host–pathogen interactions
Author(s) -
Gieffers Jens,
Tamplin Van,
Maass Matthias,
Belland Robert J.,
Caldwell Harlan D.
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00563-9
Subject(s) - mycoplasma pneumoniae , chlamydia trachomatis , chlamydia , biology , microbiology and biotechnology , pathogen , microinjection , virology , host (biology) , clone (java method) , genetics , gene , medicine , pneumonia
The Chlamydia trachomatis inclusion is fragile, rendering it incompatible to micromanipulation. We show that the Chlamydia pneumoniae inclusion differs, being resistant to micromanipulation as shown by direct microinjection of the infected host cytosol or the inclusion itself. We have used micromanipulation to clone C. pneumoniae and to free it from mycoplasma contamination.