Open AccessEffect of Urtica dioica agglutinin and Arabidopsis thaliana Chia4 chitinase on the protozoan Phytomonas françai
Author(s) -
Gomes Rocha Graça Celeste,
Nicolich Rebecca,
Romeiro Alexandre,
MargisPinheiro Márcia,
Attias Márcia,
AlvesFerreira Márcio
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00526-3
Subject(s) - chitinase , chitin , arabidopsis thaliana , biology , urtica dioica , polysaccharide , bacteria , cell wall , botany , biochemistry , enzyme , microbiology and biotechnology , gene , genetics , chitosan , mutant
The genus Phytomonas is responsible for many diseases in different crop plant species. The finding that chitin is an exposed cell surface polysaccharide in Phytomonas françai and the observation that chitinases can inhibit fungal growth raises expectations about the potential effect of plant chitinases on the P. françai cell membrane surface. The plant chitinases Urtica dioica agglutinin (UDA) and Arabidopsis thaliana Chia4 (ATCHIT4) proteins were over‐expressed in bacteria and the interaction between these proteins and P. françai surface was analyzed by immunocytochemistry. We showed that UDA and ATCHIT4 proteins can interact with surface‐exposed chitin from P. françai .