Open AccessHigh osmotic stress improves electro‐transformation efficiency of fission yeast
Author(s) -
Suga Minoru,
Kusanagi Isamu,
Hatakeyama Toyomasa
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00518-4
Subject(s) - sorbitol , osmotic shock , osmotic concentration , yeast , transformation efficiency , chemistry , osmotic pressure , biophysics , transformation (genetics) , biochemistry , biology , gene , agrobacterium
A preincubation of fission yeast cells with hyperosmotic solution improved the electro‐transformation efficiency. The efficiency increased approximately five‐fold when the cells were preincubated with 2.0 M sorbitol and 1.5 M NaCl at 30°C for 60 min before an applied high electric pulse. Losses in the efficiency of the cells after hyperosmotic stress above 2.5 M sorbitol and 2.0 M NaCl were directly related to the marked reduction of viability. The efficiency at 2.0 M sorbitol gradually increased until 60 min of the preincubation period, but longer exposure resulted in a gradual decrease. On the other hand, when the cells of the osmotic‐sensitive mutant were preincubated with isosmotic solution of 0.5 M sorbitol, the efficiency was also dramatically increased by approximately 15‐fold. These improvements in efficiency were observed in sublethal conditions of osmotic stress regardless of osmoticums and strains.