Open AccessSystematic characterization of Escherichia coli genes/ORFs affecting biofilm formation
Author(s) -
Tenorio Elizabeth,
Saeki Takashi,
Fujita Katsutoshi,
Kitakawa Madoka,
Baba Tomoya,
Mori Hirotada,
Isono Katsumi
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00507-x
Subject(s) - orfs , biofilm , escherichia coli , biology , microbiology and biotechnology , gene , open reading frame , function (biology) , bacteria , genetics , peptide sequence
To understand the nature and function of bacterial biofilm and the process of its formation, we have performed systematic screening of a complete set of Escherichia coli genes/open reading frames (ORFs) to identify those that affect biofilm development upon over‐expression. In contrast to the biofilm of strain AG1 used as a control, some of the genes/ORFs when over‐expressed led to the formation of an abnormal biofilm such as thin, mat‐like, filamentous or one easily detaching from various surfaces. Disruptants of selected genes were constructed in order to clarify their roles in the different stages of biofilm formation. Our results suggest that diverse metabolic pathways contribute to the development of biofilm.