Open AccessConstruction and characterization of a Porphyromonas gingivalis htpG disruption mutant
Author(s) -
Sweier Domenica G.,
Combs Allison,
Shelburne Charles E.,
Fenno J.Christopher,
Lopatin Dennis E.
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00506-8
Subject(s) - porphyromonas gingivalis , virulence , mutant , microbiology and biotechnology , biology , recombinant dna , polyclonal antibodies , antiserum , wild type , virulence factor , gene , bacteria , genetics , antibody
Our previous reports implicated the Hsp90 homologue (HtpG) of Porphyromonas gingivalis ( Pg ) in its virulence in periodontal disease. We investigated the role of the HtpG stress protein in the virulence of Pg . This report describes the (i) expression of a recombinant Pg HtpG (rHtpG), (ii) generation and characterization of a polyclonal rabbit anti‐ Pg rHtpG antiserum, and (iii) construction of a Pg htpG isogenic mutant and evaluation of the growth, adherence and invasion properties compared to the wild‐type parental strain. The disruption of the htpG gene did not significantly affect growth, and had no effect on Pg adherence to and invasion of cultured human cells.