Open AccessA gene transfer system for the glycopeptide producer Nonomuraea sp. ATCC39727
Author(s) -
Stinchi Sofia,
Azimonti Sara,
Donadio Stefano,
Sosio Margherita
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00490-7
Subject(s) - glycopeptide , glycopeptide antibiotic , plasmid , escherichia coli , gene transfer , gene , biology , genome , microbiology and biotechnology , antibiotics , genetics , chemistry , bacteria , staphylococcus aureus , vancomycin
The filamentous actinomycete Nonomuraea sp. ATCC39727 produces the industrially important glycopeptide antibiotic A40926. We developed a gene transfer system based on intergeneric conjugation from Escherichia coli . Analysis of the ex‐conjugants revealed that the incoming plasmid pSET152 had integrated at two sites in the Nonomuraea genome. One of these was characterized and found to be highly related to other ΦC31 attB sites described in Streptomyces spp., including the core TTS sequence, where crossover occurs. Surprisingly, pSET152 was also found in episomic form in the Nonomuraea ex‐conjugants.