Open AccessCloning and expression of the inorganic pyrophosphatase gene from the amino acid producer Brevibacterium lactofermentum ATCC 13869
Author(s) -
Ramos Angelina,
Adham Sirin A.I.,
Gil José A.
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00485-3
Subject(s) - corynebacterium glutamicum , inorganic pyrophosphatase , brevibacterium , biology , gene , microbiology and biotechnology , escherichia coli , gene expression , pyrophosphatase , gene product , fusion gene , biochemistry , genetics , bacteria , pyrophosphate , enzyme , microorganism
A 20‐kDa Brevibacterium lactofermentum protein was detected when purifying the His‐tagged FtsZ BL . The protein was identified by matrix‐assisted laser desorption/ionisation time of flight as the inorganic pyrophosphatase encoded by the ppa gene, which is present as a single copy in the genome of Corynebacterium glutamicum . The ppa gene was cloned from B. lactofermentum chromosomal DNA by polymerase chain reaction; it seemed to be an essential gene and it might represent an attractive target for drug discovery. The cloned ppa gene complemented a ppa − Escherichia coli mutant and a ppa‐gfp gene fusion revealed that the gene product mainly accumulated at the cell poles in both E. coli and B. lactofermentum .