Open AccessExpression and localization of the linear DNA plasmid‐encoded protein (RS224) in Rhizoctonia solani AG2‐2
Author(s) -
Nagasaka Atsushi,
Sasaki Atsuko,
Sasaki Toshiaki,
Yonezawa Miho,
Katsura Koji,
Hashiba Teruyoshi
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00476-2
Subject(s) - rhizoctonia solani , fusion protein , open reading frame , biology , plasmid , mycelium , escherichia coli , antiserum , microbiology and biotechnology , rhizoctonia , subcellular localization , dna , biochemistry , recombinant dna , antibody , peptide sequence , gene , genetics , botany
Expression of the linear DNA plasmid‐encoded protein (RS224) from the plant‐pathogenic fungus Rhizoctonia solani isolate H‐16, anastomosis group 2‐2, and its localization were studied. Extracts from Escherichia coli cells expressing the open reading frame (ORF) of RS224 (RS224ORF in pRS224) contain a 92‐kDa T7.Tag‐RS224orf fusion protein. Antisera raised against the fusion protein obtained from E. coli cells cross‐reacted with a 90‐kDa protein in the mycelia. To analyze the subcellular localization of the 92‐kDa protein, mycelia of R. solani were disrupted and fractionated. Antibodies against RS224 proteins specifically reacted to the mitochondrial fraction, suggesting that RS224 is localized in mitochondria.