Open AccessBldD from Streptomyces coelicolor is a non‐essential global regulator that binds its own promoter as a dimer
Author(s) -
Elliot Marie A,
Locke Troy R,
Galibois Claire M,
Leskiw Brenda K
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00474-9
Subject(s) - streptomyces coelicolor , tetramer , dimer , regulator , gene , genetics , biology , streptomyces , chemistry , biochemistry , bacteria , enzyme , organic chemistry
We have shown that the bldD gene of Streptomyces coelicolor , while required for antibiotic production and morphological differentiation, is not essential for viability. We have also demonstrated that BldD forms a higher order complex both in solution and when bound to target DNA. Purified BldD exists in three forms in solution, as a tetramer, dimer and monomer, but only in the dimeric form when bound to its own promoter/operator.