Open AccessDetection and characterization of the Gloeosporium gloeosporioides growth inhibitory compound iturin A from Bacillus subtilis strain KS03
Author(s) -
Cho SooJin,
Lee Sam Keun,
Cha Byeong Jin,
Kim Young Hwan,
Shin KwangSoo
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00329-x
Subject(s) - bacillus subtilis , lipopeptide , chromatography , chemistry , strain (injury) , molecular mass , mass spectrometry , surfactin , microbiology and biotechnology , biology , bacteria , biochemistry , enzyme , genetics , anatomy
The Bacillus subtilis strain KS03 was isolated, and identified as a biological control agent that inhibits the anthracnose disease fungus Gloeosporium gloeosporioides . The antifungal compound was purified from its culture broth through butanol extraction, diethylaminoethyl (DEAE) Sepharose CL‐6B chromatography, and preparative thin layer chromatography. Tandem mass spectrometric analyses (MS/MS), with matrix‐assisted laser desorption ionization (MALDI) time‐of‐fight/time‐of‐flight (TOF/TOF) mass spectrometry, showed that the antifungal compound was iturin A, a cyclic lipopeptide antibiotic. The major compound, with a molecular mass of 1042 Da, was identified as iturin A 2 .