Open AccessMembrane filter (pore size, 0.22–0.45 μm; thickness, 150 μm) passing‐through activity of Pseudomonas aeruginosa and other bacterial species with indigenous infiltration ability
Author(s) -
Hasegawa Hiroyuki,
Naganuma Kouta,
Nakagawa Yoji,
Matsuyama Tohey
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00327-6
Subject(s) - microbiology and biotechnology , pseudomonas aeruginosa , serratia marcescens , bacteria , chemistry , escherichia coli , membrane , listeria monocytogenes , bacillus subtilis , biology , biochemistry , genetics , gene
Bacteria growing on MF‐Millipore filters (thickness, 150 μm) passed through the underlying membrane by their infiltration activity. Bacillus subtilis , Staphylococcus aureus , Klebsiella pneumoniae , and Escherichia coli passed through a 0.45‐μm pore size filter within 48–96 h. Pseudomonas aeruginosa , Serratia marcescens , and Listeria monocytogenes passed through a 0.3‐μm pore size filter. P. aeruginosa passed through a 0.22‐μm pore size filter. The membranes which allowed passing‐through of bacteria showed normal bubble point values in the integrity test. Studies with isogenic S. marcescens mutants indicated that flagellum‐dependent motility or surface‐active exolipid were important in the passing‐through. P. aeruginosa PAO1 C strain defective in twitching motility was unable to pass through the 0.22‐μm filter. Scanning electron microscopy showed bacteria passing‐through the 0.22‐μm filter. Millipore membrane filters having well‐defined reticulate structures will be useful in the study of infiltration activity of microbes.