Open AccessThe IS 1 elements in Shigella boydii : horizontal transfer, vertical inactivation and target duplication
Author(s) -
Hsu WenBin,
Chen JiannHwa
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00319-7
Subject(s) - shigella boydii , transposition (logic) , shigella , insertion sequence , escherichia coli , biology , genetics , microbiology and biotechnology , transposable element , gene , philosophy , genome , linguistics
IS 1 (SB) and its two variants were identified as the major and minor IS 1 elements in Shigella boydii . The nucleotide sequences of IS 1 (SB), IS 1 (O157:H7) from Escherichia coli O157:H7 and IS 1 F from E. coli K12 suggest that these IS 1 elements had been horizontally transferred among S. boydii and E. coli O157:H7 and K12. The two IS 1 (SB) variants and IS 1 (O157:H7) have transposition activities 7‐ to 86‐fold less than that of IS 1 (SB), whereas IS 1 F has little transposition activity. Analysis of the flanking sequences of IS 1 (SB) and its two variants in S. boydii revealed the nature of regional specificity of the target sites and the sequence dependence of 8 and 9 bp target duplications, for which a model is presented.