Open AccessRegulation of biosynthesis and transport of aromatic amino acids in low‐GC Gram‐positive bacteria
Author(s) -
Panina Ekaterina M,
Vitreschak Alexey G,
Mironov Andrey A,
Gelfand Mikhail S
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00303-3
Subject(s) - regulon , aromatic amino acids , biochemistry , shikimate pathway , biosynthesis , biology , amino acid , gene , amino acid synthesis , transcription factor , lysine
Computational comparative techniques were applied to analysis of the aromatic amino acid regulon in Gram‐positive bacteria. A new candidate transcription regulation signal of 3‐deoxy‐ d ‐arabino‐heptulosonate‐7‐phosphate synthase and shikimate kinase genes was identified in Streptococcus and Lactococcus species. New T‐boxes were found upstream of aromatic amino acid biosynthesis and transport genes in the Bacillus / Clostridium group. The substrate specificity of proteins from the PabA/TrpG family was assigned based on metabolic reconstruction and analysis of regulatory signals and phylogenetic patterns. New candidate tryptophan transporters were identified; their specificity was predicted by analysis of T‐box regulatory sites. Comparison of all available genomes shows that regulation of genes of the aromatic amino acid biosynthesis pathway is quite labile and involves at least four regulatory systems, two at the DNA level and two more involving competition of alternative RNA secondary structures for transcription and/or translation regulation at the RNA level.