Open AccessMultiplex PCR using 16S rRNA gene‐targeted primers for the identification of bifidobacteria from human origin
Author(s) -
Mullié Catherine,
Odou MarieFrançoise,
Singer Elisabeth,
Romond MarieBénédicte,
Izard Daniel
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00245-3
Subject(s) - multiplex polymerase chain reaction , biology , polymerase chain reaction , 16s ribosomal rna , actinomycetaceae , bifidobacterium longum , multiplex , microbiology and biotechnology , bifidobacterium , variants of pcr , gene , bacteria , genetics , lactobacillus
Three multiplex polymerase chain reactions (PCRs) targeted on Bifidobacterium and related species were designed to identify human species. The selected primers yielded amplified products of various sizes, each specific for a species. Three to four pairs were gathered in one PCR reaction and their specificity under multiplex conditions was confirmed using DNA from 26 reference strains. Using this technique on unidentified faecal strains, B. bifidum , B. longum and B. breve species were commonly recovered in infants while B. adolescentis , B. catenulatum / B. pseudocatenulatum continuum and B. longum species were predominant in adults. Thus, a single PCR can provide the assignment of a strain to one these species, reducing the number of PCR reactions and hands‐on time for the identification of human isolates of bifidobacteria. Moreover, this technique is also applicable for the in situ detection of bifidobacteria in DNA extracts from human stools.