Open AccessOptimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation
Author(s) -
Legras JeanLuc,
Karst Francis
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00205-2
Subject(s) - saccharomyces cerevisiae , primer (cosmetics) , yeast , polymerase chain reaction , biology , typing , genetics , strain (injury) , genome , computational biology , chemistry , gene , organic chemistry , anatomy
A new primer pair (delta12–delta21) for polymerase chain reaction‐based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers.