Open AccessMaximization of recombinant Helicobacter pylori neutrophil activating protein production in Escherichia coli : improvement of a chemically defined medium using response surface methodology
Author(s) -
Niccolai Andrea,
Fontani Silvia,
Kapat Arnab,
Olivieri Roberto
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00184-8
Subject(s) - polyphosphate , recombinant dna , escherichia coli , chemistry , response surface methodology , helicobacter pylori , nitrogen , biochemistry , magnesium , microbiology and biotechnology , chromatography , biology , phosphate , organic chemistry , gene , genetics
Medium optimization for the production of constitutive recombinant Helicobacter pylori neutrophil activating protein (NAP) in Escherichia coli was investigated by using response surface methodology. Carbon to nitrogen ratio, concentrations of sodium polyphosphate and magnesium sulfate were considered as independent variables. The optimized medium was a chemically defined medium with a carbon to nitrogen ratio of 14.4 and with concentrations of sodium polyphosphate and magnesium sulfate about 7.1 g l −1 and 3.04 g l −1 respectively. The maximum recombinant NAP production level (1184.6 mg l −1 ) was 29.96% higher than that in control medium.