Open AccessPurification of the 67‐kDa lectin‐like glycoprotein of Trypanosoma cruzi , LLGP‐67, and its evaluation as a relevant antigen for the diagnosis of human infection
Author(s) -
Marcipar Iván S,
Welchen Elina,
Roodveldt Cintia,
Marcipar Alberto J,
Silber Ariel M
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00090-9
Subject(s) - trypanosoma cruzi , lectin , glycoprotein , antigen , chagas disease , biology , agarose , affinity chromatography , biochemistry , galactose , microbiology and biotechnology , virology , immunology , enzyme , parasite hosting , world wide web , computer science
In the present work we propose a simple method for affinity purification of the 67‐kDa lectin‐like glycoprotein (LLGP‐67) from Trypanosoma cruzi , the causative agent of Chagas’ disease. The LLGP‐67, which presents galactose binding activity and participates in the host cell recognition process, was previously purified by methods based on its interaction with galactose residues on erythrocytic membranes. We describe herein results showing that this protein can be purified from T. cruzi in a direct way using non‐derivatized agarose as a chromatographic ligand. We also demonstrate the relevance of LLGP‐67 as an antigen for human diagnosis of chagasic infection. Sensitivity and specificity for this antigen were calculated, being 98 and 98.11% respectively.