Open AccessIsolation and characterisation of a novel bacteriocin produced by Bacillus thuringiensis strain B439
Author(s) -
Ahern Melissa,
Verschueren Saskia,
Sinderen Douwe
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00086-7
Subject(s) - bacillus thuringiensis , bacteriocin , bacillus cereus , strain (injury) , peptide , bacillales , bacillaceae , microbiology and biotechnology , cereus , antimicrobial , bacillus (shape) , antimicrobial peptides , bacteria , biology , isolation (microbiology) , chemistry , bacillus subtilis , biochemistry , genetics , anatomy
Bacillus thuringiensis strain B439 produces a bacteriocin‐like inhibitory substance in its growth medium. This antimicrobial peptide, referred to as thuricin 439, acts as a bacteriocidal peptide and exhibits an apparent narrow range of inhibitory activity, essentially only affecting growth of Bacillus cereus and B. thuringiensis strains. It remains active over a relatively wide pH and temperature range, showing no loss of activity following heat treatments up to 80°C. Purification of thuricin 439 was achieved using several chromatographic steps, which resulted in the identification of two peptides with inhibitory activity. These two peptides were shown to possess identical N‐terminal sequences, but different molecular masses.