Open AccessDevelopment of PCR assays for detection of Streptococcus canis
Author(s) -
Hassan A.A,
Khan I.U,
Abdulmawjood A,
Lämmler C
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00049-1
Subject(s) - biology , polymerase chain reaction , canis , multiplex polymerase chain reaction , 16s ribosomal rna , oligonucleotide , microbiology and biotechnology , gene , ribosomal rna , genetics , paleontology
Streptococcus canis isolates, also including S. canis of artificially contaminated milk, could be identified by polymerase chain reaction (PCR) amplification using oligonucleotide primers designed according to species‐specific parts of the 16S rRNA gene and, after sequencing, according to S. canis ‐specific parts of the 16S–23S rDNA intergenic spacer region and with oligonucleotide primers detecting an internal fragment of the group G streptococcal CAMP factor gene cfg . The 16S rRNA gene‐ and CAMP factor gene cfg ‐specific oligonucleotide primers could be used together in a multiplex PCR. No cross‐reactivities could be observed with other group G streptococcal isolates or with any of the other control strains of various streptococcal species and serogroups. The PCR methods presented in this study allowed a rapid and reliable identification of S. canis and might help to improve the diagnosis of this bacterial species in animal and human infections.