Open AccessTylosin‐resistant bacteria cultivated from agricultural soil
Author(s) -
Onan Leslie J,
LaPara Timothy M
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00045-4
Subject(s) - tylosin , bacteria , biology , antibiotics , microbiology and biotechnology , antibiotic resistance , proteobacteria , 16s ribosomal rna , genetics
In this study we analyzed the numbers and types of cultivable tylosin‐resistant bacteria from six agricultural soils that differed with respect to their association with subtherapeutic antibiotic use. The proportion of tylosin‐resistant bacteria to the total number of bacteria cultivated was significantly higher (7.2–16.5%) at three sites affected by subtherapeutic antibiotic use compared to three sites unaffected by subtherapeutic antibiotic use (0.7–2.5%). We also detected differences in the types of cultivable tylosin‐resistant bacteria. At a site affected by subtherapeutic antibiotic use, we detected tylosin‐resistant bacteria from the α‐ and β‐subdivisions of Proteobacteria. In contrast, at a site unaffected by subtherapeutic use, we detected only Streptomyces ‐like (high‐G+C Gram‐positive) tylosin‐resistant bacteria. Our results suggest a link between subtherapeutic use of antibiotics and the numbers and types of antibiotic‐resistant bacteria in nearby soil. However, other factors, such as soil type and temporal variation, may have also contributed to the differences observed.