Open AccessGenetic diversity of phl D gene from 2,4‐diacetylphloroglucinol‐producing Pseudomonas spp. strains from the maize rhizosphere
Author(s) -
Picard Christine,
Bosco Marco
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00027-2
Subject(s) - biology , rhizosphere , pseudomonas , microbiology and biotechnology , restriction fragment length polymorphism , gene , genetic diversity , pseudomonadaceae , genetics , genotype , bacteria , population , demography , sociology
In biocontrol Pseudomonads, phl D is an essential gene involved in the biosynthesis of 2,4‐diacetylphloroglucinol (DAPG). Hae III restriction of amplified phl D gene, previously proposed as the most discriminant analysis, showed no polymorphism among 144 Pseudomonas strains isolated from maize roots. However, these strains fell into three statistically significant DAPG production level groups. phl D sequences of 13 strains belonging to the three DAPG groups revealed a Ksp I restriction site only in good DAPG‐producing strains. This result was confirmed on the 144 strains, 82 of which were identified as good‐DAPG producers by both biochemical and amplified phl D Ksp I restriction analysis. They are candidates as potential biocontrol agents.