Open AccessMulti locus fingerprinting of Listeria monocytogenes by sequence‐specific labeling of DNA probes combined with array hybridization
Author(s) -
Rudi Knut,
Katla Tone,
Naterstad Kristine
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00026-0
Subject(s) - multilocus sequence typing , biology , listeria monocytogenes , dna profiling , listeria , oligonucleotide , typing , locus (genetics) , dna , polymerase chain reaction , genetics , hybridization probe , dna sequencing , gene , microbiology and biotechnology , bacteria , genotype
We have developed an alternative multi locus sequence typing (MLST) approach that targets the variable genetic changes directly in a DNA array format. Our approach is based on DNA array hybridization in combination with sequence‐specific labeling of oligonucleotide probes. Listeria monocytogenes was chosen for the development and evaluation of the assay. The genes hlyA , iap , flaA , inlA and actA were targeted. Twenty‐nine suitable probe regions were identified within these genes. The DNA array results from 32 different strains were compared to serotype and amplified fragment length polymorphism data. This comparison showed that our DNA array method gave good discrimination between the strains analyzed. In conclusion, the DNA array‐based MLST method is a promising tool for fingerprint bacteria.