Open AccessCloning and expression of hyaluronate lyase genes of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus 1
Author(s) -
Takao Ayuko
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00023-5
Subject(s) - biology , streptococcus intermedius , microbiology and biotechnology , streptococcaceae , gene , open reading frame , streptococcus pneumoniae , bacteroidaceae , streptococcus , peptide sequence , bacteria , genetics , antibiotics
Hyaluronate lyase (HAase) genes of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus were isolated. In S. constellatus subsp. constellatus , the deduced amino acid sequence of HAase was most similar to that of S. intermedius (68%), whereas the enzyme of S. intermedius was most similar to that of S. pneumoniae (72%). Upstream of the HAase gene on the opposite strands, an open reading frame of a putative glutathione peroxidase started in S. intermedius , and this arrangement was similar to that in S. pneumoniae but unlike that in S. constellatus subsp. constellatus . Cell lysates of Escherichia coli carrying each streptococcal gene showed HAase activity, demonstrating that each cloned gene actually coded for HAase.