Open AccessCloning and expression of a putative cyclodextrin glucosyltransferase from the symbiotically competent cyanobacterium Nostoc sp. PCC 9229
Author(s) -
Wouters Johanna,
Bergman Birgitta,
Janson Sven
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(02)01204-1
Subject(s) - nostoc , biology , glucosyltransferase , gene , cloning (programming) , cyanobacteria , sequence analysis , gene expression , microbiology and biotechnology , biochemistry , genetics , bacteria , computer science , programming language
A polymerase chain reaction‐based method was used to isolate a Nostoc sp. PCC 9229 cDNA from infected glands of Gunnera chilensis . The complete gene sequence was isolated from a genomic Nostoc sp. PCC 9229 library. Sequence analysis showed 84% amino acid similarity to a putative cyclodextrin glycosyltransferase from Nostoc sp. PCC 7120 and the gene was therefore termed cgt . Southern blot revealed that the cgt gene was present in symbiotically competent cyanobacteria. The cgt gene was expressed in free‐living nitrogen‐fixing cultures in light or in darkness when supplemented with fructose. This is the first expression analysis of a cgt gene from a cyanobacterium.