Open AccessInvolvement of oxidative stress in the regulation of H 2 S production during ultradian metabolic oscillation of Saccharomyces cerevisiae
Author(s) -
Kwak WieJong,
Kwon GiSeok,
Jin Ingnyol,
Kuriyama Hiroshi,
Sohn HoYong
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(02)01198-9
Subject(s) - chemostat , oxidative stress , ultradian rhythm , oxidative phosphorylation , saccharomyces cerevisiae , biochemistry , glutathione , biology , chemistry , biophysics , yeast , enzyme , endocrinology , circadian rhythm , genetics , bacteria
Periodic evolution of H 2 S during aerobic chemostat culture of Saccharomyces cerevisiae resulted in ultradian metabolic oscillation via periodic inhibition of respiratory activity. To understand the nature of periodic H 2 S evolution, we investigated whether oxidative stress is associated with H 2 S production. The cellular oxidative states represented by intracellular level of lipid peroxides oscillated out of phase with the oscillation of dissolved O 2 . Pulse addition of antioxidant, oxidative agent or inhibitor of antioxidation enzymes perturbed metabolic oscillation producing changes in H 2 S evolution. Analysis of H 2 S production profiles during perturbation of oscillation revealed that the amount of H 2 S production is closely linked with cellular oxidative states. Based on these results and our previous reports, we suggest that oxidative stresses result in periodic depletion of glutathione and cysteine, which in turn causes stimulation of the sulfate assimilation pathway and H 2 S production.