Open AccessProperties of a trichlorodibenzo‐ p ‐dioxin‐dechlorinating mixed culture with a Dehalococcoides as putative dechlorinating species
Author(s) -
Ballerstedt Hendrik,
Hantke Janina,
Bunge Michael,
Werner Birgit,
Gerritse Jan,
Andreesen Jan R,
Lechner Ute
Publication year - 2004
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1016/s0168-6496(03)00282-4
Subject(s) - dehalococcoides , reductive dechlorination , biology , bacteria , microbiology and biotechnology , dehalogenase , halogenation , enrichment culture , desulfovibrio , 16s ribosomal rna , environmental chemistry , biodegradation , ecology , chemistry , organic chemistry , vinyl chloride , genetics , copolymer , polymer
An anaerobic mixed culture enriched over 16 transfers (1/10) from Saale river sediment reductively dehalogenated 1,2,4‐ and 1,2,3‐trichlorodibenzo‐ p ‐dioxin (TrCDD) to di‐ and monochlorinated congeners in the presence of pyruvate (or lactate) and fumarate as cosubstrates. Besides TrCDD, tetrachloroethene and 1,2,3‐trichlorobenzene were dechlorinated. Dioxin dehalogenation was sensitive to pasteurization, but was not remarkably influenced by inhibitors of methanogens, sulfate‐reducing bacteria or Gram‐positive bacteria. The rate of 1,3‐dichlorodibenzo‐ p ‐dioxin formation increased with rising initial concentrations of 1,2,4‐TrCDD (1–250 μM) from 0.05 to 5.4 μmol l −1 day −1 . Two isolates, belonging to Sulfurospirillum and Trichococcus , did not show reductive dehalogenation. 16S rDNA‐targeted methods further revealed the presence of Acetobacterium , Desulfitobacterium , Desulfuromonas and Dehalococcoides . Nested polymerase chain reaction (PCR) indicated the presence of Dehalococcoides in highest most probable number (MPN) dilutions that were positive for dioxin dechlorination.