Open AccessFungal modification of the hydroxyl radical detector coumarin‐3‐carboxylic acid
Author(s) -
Iakovlev Andrei,
Broberg Anders,
Stenlid Jan
Publication year - 2003
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1016/s0168-6496(03)00213-7
Subject(s) - malonic acid , carboxylic acid , coumarin , hydroxyl radical , incubation , radical , biology , chromatography , organic chemistry , biochemistry , chemistry
The feasibility of using coumarin‐3‐carboxylic acid (3‐CCA) for detection of hydroxyl radicals in pure cultures of wood‐decaying fungi was tested. Fungi were incubated on a 3‐CCA‐containing medium. The transformation of 3‐CCA to the fluorescent hydroxyl radical detector substance 7‐hydroxycoumarin‐3‐carboxylic acid and other compounds was studied by chromatographic and spectroscopic techniques. During incubation of all tested fungi, a small fraction of the 3‐CCA was hydroxylated to 7‐hydroxycoumarin‐3‐carboxylic acid and a major fraction of the 3‐CCA was metabolized by fungi to 2‐(2‐hydroxybenzyl)malonic acid. In most cultures the concentration of 3‐CCA was below detection limit at the end of incubation. The fungal metabolism was suggested to be involved in the formation of 2‐(2‐hydroxybenzyl)malonic acid from 3‐CCA, consequently making this method of hydroxyl radical detection less suitable to use on cultures of wood‐decaying fungi.