Open AccessCharacteristics of Na + ‐dependent respiratory chain in Vibrio anguillarum , a fish pathogen, in comparison with other marine Vibrio s
Author(s) -
FujiwaraNagata Erina,
Kogure Kazuhiro,
KitaTsukamoto Kumiko,
Wada Minoru,
Eguchi Mitsuru
Publication year - 2003
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1016/s0168-6496(03)00031-x
Subject(s) - vibrio anguillarum , vibrio alginolyticus , biology , microbiology and biotechnology , vibrio , seawater , vibrionaceae , serotype , pathogen , bacteria , ecology , genetics
The activity of membrane‐bound NADH oxidase of Vibrio anguillarum M93 (serotype J‐O‐1), which causes vibriosis in freshwater area was activated by Na + in the same manner as other marine Vibrio s. However, in addition to Na + , K + was also found to positively enhance the NADH oxidase activity of strain M93. This tendency has not been recognized in other marine Vibrio s. Furthermore, the Na + ‐dependent NADH oxidase of strain M93 required less Na + (0.1 M) for its maximum activity than those of other Vibrio s such as Vibrio alginolyticus and ‘ Vibrio angustum ’ S14, which were in the range of 0.4 M NaCl, similar as seawater. Destruction of H + motive force by a proton conductor carbonylcyanide m ‐chlorophenylhydrazone (CCCP) revealed high dependency of V. anguillarum on the primary H + pump. Even at pH 8.5, V. anguillarum strains other than serotype O‐4 could not grow well with the addition of CCCP. In contrast, marine‐type Vibrio s such as V. alginolyticus and V. angustum S14 can grow well at pH 8.5 even with the addition of CCCP. The lower requirement for Na + in V. anguillarum probably reflects the salinity of their original habitats.