Open AccessCD62L expression level determines the cell fate of myeloid progenitors
Author(s) -
Yusuke Ito,
Fumio Nakahara,
Yuki Kagoya,
Mineo Kurokawa
Publication year - 2021
Publication title -
stem cell reports
Language(s) - English
Resource type - Journals
ISSN - 2213-6711
DOI - 10.1016/j.stemcr.2021.10.012
Subject(s) - progenitor cell , biology , myeloid , haematopoiesis , microbiology and biotechnology , progenitor , monocyte , population , cellular differentiation , myelopoiesis , immunology , stem cell , genetics , gene , demography , sociology
Hematopoietic cells differentiate through several progenitors in a hierarchical manner, and recent single-cell analyses have revealed substantial heterogeneity within each progenitor. Although common myeloid progenitors (CMPs) are defined as a multipotent cell population that can differentiate into granulocyte-monocyte progenitors (GMPs) and megakaryocyte-erythrocyte progenitors (MEPs), and GMPs generate neutrophils and monocytes, these myeloid progenitors must contain some lineage-committed progenitors. Through gene expression analysis at single-cell levels, we identified CD62L as a marker to reveal the heterogeneity. We confirmed that CD62L-negative CMPs represent "bona fide" CMPs, whereas CD62L-high CMPs are mostly restricted to GMP potentials both in mice and humans. In addition, we identified CD62L-negative GMPs as the most immature subsets in GMPs and Ly6C + CD62L-intermediate and Ly6C + CD62L-high GMPs are skewed to neutrophil and monocyte differentiation in mice, respectively. Our findings contribute to more profound understanding about the mechanism of myeloid differentiation.