Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type | Zendy

Jeoung Eun Lee | Zendy; Ji Yoon Lee | Zendy; ChangHwan Park | Zendy; Jin Hee Eum | Zendy; Soo Kyung Jung | Zendy; AReum Han | Zendy; DongWon Seol | Zendy; Jin Saem Lee | Zendy; Hyun Soo Shin | Zendy; Jung Ho Im | Zendy; Taehoon Chun | Zendy; Kyungsoo Ha | Zendy; Deok Rim Heo | Zendy; Tae Ki Yoon | Zendy; Dong Ryul Lee | Zendy

Open Access

Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type

Author(s) -

Jeoung Eun Lee,

Ji Yoon Lee,

ChangHwan Park,

Jin Hee Eum,

Soo Kyung Jung,

AReum Han,

DongWon Seol,

Jin Saem Lee,

Hyun Soo Shin,

Jung Ho Im,

Taehoon Chun,

Kyungsoo Ha,

Deok Rim Heo,

Tae Ki Yoon,

Dong Ryul Lee

Publication year - 2020

Publication title -

stem cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.207

H-Index - 76

ISSN - 2213-6711

DOI - 10.1016/j.stemcr.2020.05.005

Subject(s) - somatic cell nuclear transfer , biology , induced pluripotent stem cell , embryonic stem cell , somatic cell , stem cell , microbiology and biotechnology , cord blood , immunology , human leukocyte antigen , regenerative medicine , antigen , blastocyst , genetics , embryo , embryogenesis , gene

Human pluripotent stem cells (PSCs) through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryopreserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine.

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