Identification and Single-Cell Functional Characterization of an Endodermally Biased Pluripotent Substate in Human Embryonic Stem Cells | Zendy

Thomas F. Allison | Zendy; Andrew J. H. Smith | Zendy; Konstantinos Anastassiadis | Zendy; Jackie SloaneStanley | Zendy; Veronica Biga | Zendy; Dylan Stavish | Zendy; James O.S. Hackland | Zendy; Shan Sabri | Zendy; Justin Langerman | Zendy; Mark Jones | Zendy; Kathrin Plath | Zendy; Daniel Coca | Zendy; Ivana Barbaric | Zendy; Paul J. Gokhale | Zendy; Peter W. Andrews | Zendy

Open Access

Identification and Single-Cell Functional Characterization of an Endodermally Biased Pluripotent Substate in Human Embryonic Stem Cells

Author(s) -

Thomas F. Allison,

Andrew J. H. Smith,

Konstantinos Anastassiadis,

Jackie SloaneStanley,

Veronica Biga,

Dylan Stavish,

James O.S. Hackland,

Shan Sabri,

Justin Langerman,

Mark Jones,

Kathrin Plath,

Daniel Coca,

Ivana Barbaric,

Paul J. Gokhale,

Peter W. Andrews

Publication year - 2018

Publication title -

stem cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.207

H-Index - 76

ISSN - 2213-6711

DOI - 10.1016/j.stemcr.2018.04.015

Subject(s) - biology , embryonic stem cell , gata6 , stem cell , endoderm , induced pluripotent stem cell , embryoid body , microbiology and biotechnology , cellular differentiation , cloning (programming) , inner cell mass , transcription factor , genetics , gene , embryogenesis , blastocyst , embryo , computer science , programming language

Human embryonic stem cells (hESCs) display substantial heterogeneity in gene expression, implying the existence of discrete substates within the stem cell compartment. To determine whether these substates impact fate decisions of hESCs we used a GFP reporter line to investigate the properties of fractions of putative undifferentiated cells defined by their differential expression of the endoderm transcription factor, GATA6, together with the hESC surface marker, SSEA3. By single-cell cloning, we confirmed that substates characterized by expression of GATA6 and SSEA3 include pluripotent stem cells capable of long-term self-renewal. When clonal stem cell colonies were formed from GATA6-positive and GATA6-negative cells, more of those derived from GATA6-positive cells contained spontaneously differentiated endoderm cells than similar colonies derived from the GATA6-negative cells. We characterized these discrete cellular states using single-cell transcriptomic analysis, identifying a potential role for SOX17 in the establishment of the endoderm-biased stem cell state.

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