Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction | Zendy

Xin Zhao | Zendy; Haodong Chen | Zendy; Dan Xiao | Zendy; Huaxiao Yang | Zendy; Ilanit Itzhaki | Zendy; Xulei Qin | Zendy; Tony Chour | Zendy; Aitor Aguirre | Zendy; Kim A. Lehmann | Zendy; Youngkyun Kim | Zendy; Praveen Shukla | Zendy; Alexandra Holmström | Zendy; Joe Z. Zhang | Zendy; Yan Zhuge | Zendy; B Ndoye | Zendy; MingTao Zhao | Zendy; Evgenios Neofytou | Zendy; WolframHubertus Zimmermann | Zendy; Mohit Jain | Zendy; Joseph C. Wu | Zendy

Open Access

Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction

Author(s) -

Xin Zhao,

Haodong Chen,

Dan Xiao,

Huaxiao Yang,

Ilanit Itzhaki,

Xulei Qin,

Tony Chour,

Aitor Aguirre,

Kim A. Lehmann,

Youngkyun Kim,

Praveen Shukla,

Alexandra Holmström,

Joe Z. Zhang,

Yan Zhuge,

B Ndoye,

MingTao Zhao,

Evgenios Neofytou,

WolframHubertus Zimmermann,

Mohit Jain,

Joseph C. Wu

Publication year - 2018

Publication title -

stem cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.207

H-Index - 76

ISSN - 2213-6711

DOI - 10.1016/j.stemcr.2018.01.002

Subject(s) - biology , induced pluripotent stem cell , myocardial infarction , stem cell , human heart , non human primate , primate , medicine , cardiology , microbiology and biotechnology , neuroscience , embryonic stem cell , genetics , evolutionary biology , gene

Non-human primates (NHPs) can serve as a human-like model to study cell therapy using induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). However, whether the efficacy of NHP and human iPSC-CMs is mechanistically similar remains unknown. To examine this, RNU rats received intramyocardial injection of 1 × 10 7 NHP or human iPSC-CMs or the same number of respective fibroblasts or PBS control (n = 9-14/group) at 4 days after 60-min coronary artery occlusion-reperfusion. Cardiac function and left ventricular remodeling were similarly improved in both iPSC-CM-treated groups. To mimic the ischemic environment in the infarcted heart, both cultured NHP and human iPSC-CMs underwent 24-hr hypoxia in vitro. Both cells and media were collected, and similarities in transcriptomic as well as metabolomic profiles were noted between both groups. In conclusion, both NHP and human iPSC-CMs confer similar cardioprotection in a rodent myocardial infarction model through relatively similar mechanisms via promotion of cell survival, angiogenesis, and inhibition of hypertrophy and fibrosis.

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