Isolation and Comparative Transcriptome Analysis of Human Fetal and iPSC-Derived Cone Photoreceptor Cells | Zendy

Emily Welby | Zendy; Jörn Lakowski | Zendy; Valentina Di Foggia | Zendy; Dimitri Budinger | Zendy; Anai Gonzalez-Cordero | Zendy; Aaron T. L. Lun | Zendy; Michael P. Epstein | Zendy; Aara Patel | Zendy; Elisa Cuevas | Zendy; Kamil Kruczek | Zendy; Arifa Naeem | Zendy; Federico Minneci | Zendy; Michael Hubank | Zendy; David T. Jones | Zendy; John C. Marioni | Zendy; Robin R. Ali | Zendy; Jane C. Sowden | Zendy

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Isolation and Comparative Transcriptome Analysis of Human Fetal and iPSC-Derived Cone Photoreceptor Cells

Author(s) -

Emily Welby,

Jörn Lakowski,

Valentina Di Foggia,

Dimitri Budinger,

Anai Gonzalez-Cordero,

Aaron T. L. Lun,

Michael P. Epstein,

Aara Patel,

Elisa Cuevas,

Kamil Kruczek,

Arifa Naeem,

Federico Minneci,

Michael Hubank,

David T. Jones,

John C. Marioni,

Robin R. Ali,

Jane C. Sowden

Publication year - 2017

Publication title -

stem cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.207

H-Index - 76

ISSN - 2213-6711

DOI - 10.1016/j.stemcr.2017.10.018

Subject(s) - biology , opsin , transplantation , stem cell , transcriptome , microbiology and biotechnology , retina , cell sorting , retinal , cell , genetics , gene , gene expression , neuroscience , medicine , rhodopsin , botany

Loss of cone photoreceptors, crucial for daylight vision, has the greatest impact on sight in retinal degeneration. Transplantation of stem cell-derived L/M-opsin cones, which form 90% of the human cone population, could provide a feasible therapy to restore vision. However, transcriptomic similarities between fetal and stem cell-derived cones remain to be defined, in addition to development of cone cell purification strategies. Here, we report an analysis of the human L/M-opsin cone photoreceptor transcriptome using an AAV2/9.pR2.1:GFP reporter. This led to the identification of a cone-enriched gene signature, which we used to demonstrate similar gene expression between fetal and stem cell-derived cones. We then defined a cluster of differentiation marker combination that, when used for cell sorting, significantly enriches for cone photoreceptors from the fetal retina and stem cell-derived retinal organoids, respectively. These data may facilitate more efficient isolation of human stem cell-derived cones for use in clinical transplantation studies.

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