Small RNA Sequencing Reveals Dlk1-Dio3 Locus-Embedded MicroRNAs as Major Drivers of Ground-State Pluripotency | Zendy

Sharif Moradi | Zendy; Ali SharifiZarchi | Zendy; Amirhossein Ahmadi | Zendy; Sepideh Mollamohammadi | Zendy; Alexander Stubenvoll | Zendy; Stefan Günther | Zendy; Ghasem Hosseini Salekdeh | Zendy; Sassan Asgari | Zendy; Thomas Braun | Zendy; Hossein Baharvand | Zendy

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Small RNA Sequencing Reveals Dlk1-Dio3 Locus-Embedded MicroRNAs as Major Drivers of Ground-State Pluripotency

Author(s) -

Sharif Moradi,

Ali SharifiZarchi,

Amirhossein Ahmadi,

Sepideh Mollamohammadi,

Alexander Stubenvoll,

Stefan Günther,

Ghasem Hosseini Salekdeh,

Sassan Asgari,

Thomas Braun,

Hossein Baharvand

Publication year - 2017

Publication title -

stem cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.207

H-Index - 76

ISSN - 2213-6711

DOI - 10.1016/j.stemcr.2017.10.009

Subject(s) - biology , embryonic stem cell , microrna , psychological repression , microbiology and biotechnology , genetics , rex1 , gene , gene expression , induced pluripotent stem cell

Ground-state pluripotency is a cell state in which pluripotency is established and maintained through efficient repression of endogenous differentiation pathways. Self-renewal and pluripotency of embryonic stem cells (ESCs) are influenced by ESC-associated microRNAs (miRNAs). Here, we provide a comprehensive assessment of the "miRNome" of ESCs cultured under conditions favoring ground-state pluripotency. We found that ground-state ESCs express a distinct set of miRNAs compared with ESCs grown in serum. Interestingly, most "ground-state miRNAs" are encoded by an imprinted region on chromosome 12 within the Dlk1-Dio3 locus. Functional analysis revealed that ground-state miRNAs embedded in the Dlk1-Dio3 locus (miR-541-5p, miR-410-3p, and miR-381-3p) promoted pluripotency via inhibition of multi-lineage differentiation and stimulation of self-renewal. Overall, our results demonstrate that ground-state pluripotency is associated with a unique miRNA signature, which supports ground-state self-renewal by suppressing differentiation.

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