Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis | Zendy

Aleeza Farrukh | Zendy; Felipe Ortega | Zendy; Wenqiang Fan | Zendy; Nicolás Marichal | Zendy; Julieta I. Paez | Zendy; Benedikt Berninger | Zendy; Aránzazu del Campo | Zendy; Marcelo Salierno | Zendy

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Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis

Author(s) -

Aleeza Farrukh,

Felipe Ortega,

Wenqiang Fan,

Nicolás Marichal,

Julieta I. Paez,

Benedikt Berninger,

Aránzazu del Campo,

Marcelo Salierno

Publication year - 2017

Publication title -

stem cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.207

H-Index - 76

ISSN - 2213-6711

DOI - 10.1016/j.stemcr.2017.09.002

Subject(s) - neurogenesis , embryonic stem cell , progenitor cell , neural stem cell , laminin , microbiology and biotechnology , self healing hydrogels , biology , progenitor , stem cell , polylysine , integrin , cell adhesion , cell , extracellular matrix , chemistry , biochemistry , organic chemistry , gene

Engineering of biomaterials with specific biological properties has gained momentum as a means to control stem cell behavior. Here, we address the effect of bifunctionalized hydrogels comprising polylysine (PL) and a 19-mer peptide containing the laminin motif IKVAV (IKVAV) on embryonic and adult neuronal progenitor cells under different stiffness regimes. Neuronal differentiation of embryonic and adult neural progenitors was accelerated by adjusting the gel stiffness to 2 kPa and 20 kPa, respectively. While gels containing IKVAV or PL alone failed to support long-term cell adhesion, in bifunctional gels, IKVAV synergized with PL to promote differentiation and formation of focal adhesions containing β 1 -integrin in embryonic cortical neurons. Furthermore, in adult neural stem cell culture, bifunctionalized gels promoted neurogenesis via the expansion of neurogenic clones. These data highlight the potential of synthetic matrices to steer stem and progenitor cell behavior via defined mechano-adhesive properties.

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