Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering | Zendy

Molly Lowndes | Zendy; Michael Rotherham | Zendy; Joshua C. Price | Zendy; Alicia J. El Haj | Zendy; Shukry J. Habib | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering

Author(s) -

Molly Lowndes,

Michael Rotherham,

Joshua C. Price,

Alicia J. El Haj,

Shukry J. Habib

Publication year - 2016

Publication title -

stem cell reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.207

H-Index - 76

ISSN - 2213-6711

DOI - 10.1016/j.stemcr.2016.06.004

Subject(s) - wnt signaling pathway , biology , embryonic stem cell , microbiology and biotechnology , stem cell , stem cell niche , tissue engineering , wnt3a , niche , adult stem cell , signal transduction , progenitor cell , biochemistry , genetics , gene

The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research