Open AccessGeneration of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line
Author(s) -
Benjamin Schmid,
Kennie R. Prehn,
Natakarn Nimsanor,
Blanca Irene Aldana Garcia,
Ulla Poulsen,
Ida Jørring,
Mikkel A. Rasmussen,
Christian Clausen,
Ulrike A. MauHolzmann,
Sarayu Ramakrishna,
Ravi Muddashetty,
Rachel Steeg,
K. Bruce,
Peter Mackintosh,
Andreas Ebneth,
Bjørn Holst,
Alfredo CabreraSocorro
Publication year - 2019
Publication title -
stem cell research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.654
H-Index - 65
eISSN - 1876-7753
pISSN - 1873-5061
DOI - 10.1016/j.scr.2018.11.010
Subject(s) - apolipoprotein e , biology , induced pluripotent stem cell , allele , genotype , genetics , gene knockin , gene , knockout mouse , crispr , disease , medicine , embryonic stem cell
Alzheimer's disease (AD) is the most frequent neurodegenerative disease amongst the elderly. The SNPs rs429358 and rs7412 in the APOE gene are the most common risk factor for sporadic AD, and there are three different alleles commonly referred to as APOE-ε2, APOE-ε3 and APOE-ε4. Induced pluripotent stem cells (iPSCs) hold great promise to model AD as such cells can be differentiated in vitro to the required cell type. Here we report the use of CRISPR/Cas9 technology employed on iPSCs from a healthy individual with an APOE-ε3/ε4 genotype to obtain isogenic APOE-ε2/ε2, APOE-ε3/ε3, APOE-ε4/ε4 lines as well as an APOE-knock-out line.
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